Sir Chris Hoy was beaten into second place in the keirin at the Track World Cup in Kazakhstan.
The four-time Olympic champion, who missed last month's European Championships through illness, came second to Christos Volikakis of Greece in his preferred event.
Bolton-born Jason Kenny failed to qualify for the final as his miserable run on the track continued.
Elsewhere, European champion Ed Clancy missed out on a medal in the omnium.
The Barnsley rider finished fourth in the six-discipline event behind Germany's Roger Kluge.
Dani King was lying fourth overnight in the corresponding women's event, which concludes with three events tomorrow.
In the women's sprint, Jess Varnish and Becky James were eliminated during the first knockout round as world champion Anna Meares of Australia was beaten to gold by Ukraine's Lyubov Shulika.
Hoy, Kenny and Matt Crampton will ride in the men's sprint in Sunday's final day of competition in Astana, with King competing in the remaining three omnium events.